脫氧核糖核酸酶Ⅱ（豬脾）資料說明

【資料簡介】

英文名稱：DNase II ；Deoxyribonuclease Ⅱ(porcine spleen)；Deoxyribonucleate 3′-oligonucleotido-hydrolase
其他名稱：去氧細胞核酸酶；氧核糖核酸-5ˊ-寡核苷酸水解酶；無核糖核酸酶；脫氧核糖核酸去聚合酶；脫羥核糖核酸酶(豬胰)；除氧細胞核酸酶(豬胰) ；DNA酶
CAS號：9025-64-3
級別：BR 
類型：Type IV
活力：2000～6000 units/mg
活力定義：One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃； [Mg2+] = 0.83 mM
性狀(以下信息僅供參考)：白色或類白色凍干粉。essentially salt-free。為雙鏈特異性內切核酸酶，能降解雙鏈DNA成為具有5ˊ-磷酸及3ˊ-OH末端的寡脫氧核糖核苷酸。當有一價錳離子存在時，亦能降解單鏈DNA。溶于水，微溶于30％乙醇和丙酮。酶反應：脫氧核糖核酸→二核苷酸5′磷酸+寡聚核苷酸5′磷酸zui適pH 7.8。吸光系數(E1%280)11.1。激活劑為二價金屬離子，zui大激活可以從鎂(Mg2+)+鈣(Ca2+)得到。抑制劑有EDTA和十二烷基硫酸鈉。可加5mmol/L鈣(Ca2+)
用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)用于分子生物學研究。
保存：-20℃
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