Anti-CD34抗體說明書

【資料簡介】

 Anti-CD34抗體說明書

Catalog Number :   Rs-0646 R

 

Quantity size :   0.1ml （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

 

Background:    The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. 

 Anti-CD34抗體說明書

 

Specificity :   · anti-CD34 is a rabbit polyclonal antibody unconjugated

            · specific for CD34 of hu, rat, mo, cow, pig, dog, Rb

            · epitope mapping in the C-terminalse of CD34 of hu, rat, mo, cow, pig, dog, Rb

· use for western blotting, elisa, immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 34kDa

               

Application:   ·Western blotting 1:100-500

             ·Immunohistochemistry 1:100-500

             · ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage:    Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

 Anti-CD34抗體說明書