Agarose plug:
1% agarose dissolved in 1x Resolving gel buffer.
（I make 50 ml, keep melting it as I need it, and re-adding water to maintain agarose conc.）
Resolving gel: 24 ml of a 9% gel
5.4 ml 40% acrylamide/bisacrylamide （29:1 mix）
3 ml 8x Resolving gel buffer
15.6 ml water
12 µl TEMED
60 µl 20% ammonium persulfate
Stacking gel: 8 ml
1 ml 40% acrylamide/bisacrylamide （29:1 mix）
2 ml 4x Stacking gel buffer
5 ml water
8 µl TEMED
21.6 µl 20% ammonium persulfate
After flash spinning the samples, load into the wells.
Be sure to use markers.
We use 15 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly.
Run with constant current （35 - 37 mA with voltage set at > 300 V）.
Usual running time is about 2.5 hr.
After flash spinning the samples, load into the wells.
Be sure to use markers.
We use 15 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly.
Run with constant current （35 - 37 mA with voltage set at > 300 V）.
Usual running time is about 2.5 hr.
| Anti-TRP2/FITC 熒光素標記*酶相關蛋白2抗體IgG |
| Anti-Trx/FITC 熒光素標記硫氧還蛋白抗體IgG |
| Anti-TSARG3/FITC 熒光素標記生精細胞凋亡相關基因3抗體IgG |
| Anti-TSARG4/FITC 熒光素標記生精細胞凋亡相關基因4抗體IgG |
| Anti-TSARG6/FITC 熒光素標記生精細胞凋亡相關基因6抗體IgG |
| Anti-TSARG7/FITC 熒光素標記精子發生相關的新基因抗體IgG |
| Anti-TSFP1/SP1 /FITC 熒光素標記SP1轉錄生長因子抗體IgG |
| Anti-tsg101/FITC 熒光素標記tsg101抗體IgG |
| Anti-TSHR/FITC 熒光素標記促甲狀腺素受體抗體IgG |
| Anti-TSHR（CT）/FITC 熒光素標記促甲狀腺素受體抗體（C端）IgG |
| Anti-TSHR /FITC 熒光素標記促甲狀腺素受體抗體IgG |
| Anti-TSHR（NT）/FITC 熒光素標記促甲狀腺素受體抗體（N端）IgG |
| Anti-TSLC1/FITC 熒光素標記肺癌腫瘤阻抑基因1抗體IgG |
| Anti-TSLP/FITC 熒光素標記淋巴細胞生成素-1抗體IgG |
| Anti-TTF-1/FITC 熒光素標記甲狀腺核轉錄因子-1/甲狀腺特異增強子結合蛋白抗體IgG |
| Anti-TTF-2/FITC 熒光素標記甲狀腺轉錄因子-2抗體IgG |
| Anti-Tubb3/FITC 熒光素標記β微管蛋白-Ⅲ抗體IgG |
| Anti-Tubulin-α/FITC 熒光素標記微管蛋白α抗體IgG |
| Anti-Tubulin-β/FITC 熒光素標記微管蛋白抗體IgG |
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